People's Perception of Well-Being during the COVID-19 Pandemic: A Case Study in Japan.

This study aims to examine people's perception of well-being during the COVID-19 pandemic in Japan and quantitatively clarify key factors towards realizing evidence-based policymaking. In March 2022, 400 participants responded to a survey conducted through Rakuten Insight. The authors applied an ordinal logistic regression (OLR), followed by principal component analysis (PCA), to create a new compound indicator (CI) to represent people's perception of well-being during the pandemic in addition to ordinary least squares (OLS) regression with a forward-backward stepwise selection method, where the dependent variable is the principal component score of the first principal component (PC1), while the independent variables are the same as the abovementioned OLR. Consequently, while analyzing OLR, some independent variables showed statistical significance, while the CI provided an option to grasp people's perception of well-being. Furthermore, family structure was statistically significant in all cases of OLR and OLS. Moreover, in terms of the standardized coefficients (beta) of OLS, the family structure had the greatest impact on the CI. Based on the study results, the authors advocate that the Japanese government should pay more attention to single-person households affected by the COVID-19 pandemic.

Resilience adaptive capacity wheel: Challenges for hotel stakeholders in the event of a tsunami during the Tokyo Olympics

International tourism continues to grow with ten years of consecutive growth, excluding an expected period of decline in 2020 due to the COVID-19 Pandemic. Since 2011, tourism in Japan has seen a sharp rise due to the relaxation of visa requirements, as part of the Japanese government's strategy to revitalize the national economy since the 2011 Great East Japan Earthquake. This number is expected to rise during the 2020–2021 Tokyo Olympics. However increased tourism also exposes more people to Japan's numerous natural hazard vulnerabilities, such as earthquakes and tsunamis. Several venues for the Tokyo Olympics are located in Kanagawa Prefecture, which has a past history of powerful earthquakes and devastating tsunamis. This research examines how hotels along Kanagawa's Sagami Bay, a popular tourism destination prepare for a tsunami event. For this research, we've developed a resilience adaptive capacity wheel to examine elements of public-private collaboration for disaster preparedness.

A diagnostic host response biosignature for COVID-19 from RNA profiling of nasal swabs and blood.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease-19 (COVID-19), has emerged as the cause of a global pandemic. We used RNA sequencing to analyze 286 nasopharyngeal (NP) swab and 53 whole-blood (WB) samples from 333 patients with COVID-19 and controls. Overall, a muted immune response was observed in COVID-19 relative to other infections (influenza, other seasonal coronaviruses, and bacterial sepsis), with paradoxical down-regulation of several key differentially expressed genes. Hospitalized patients and outpatients exhibited up-regulation of interferon-associated pathways, although heightened and more robust inflammatory responses were observed in hospitalized patients with more clinically severe illness. Two-layer machine learning-based host classifiers consisting of complete (>1000 genes), medium (<100), and small (<20) gene biomarker panels identified COVID-19 disease with 85.1-86.5% accuracy when benchmarked using an independent test set. SARS-CoV-2 infection has a distinct biosignature that differs between NP swabs and WB and can be leveraged for COVID-19 diagnosis.

SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood.

Given the limited availability of serological testing to date, the seroprevalence of SARS-CoV-2-specific antibodies in different populations has remained unclear. Here, we report very low SARS-CoV-2 seroprevalence in two San Francisco Bay Area populations. Seroreactivity was 0.26% in 387 hospitalized patients admitted for non-respiratory indications and 0.1% in 1,000 blood donors in early April 2020. We additionally describe the longitudinal dynamics of immunoglobulin-G (IgG), immunoglobulin-M (IgM), and in vitro neutralizing antibody titers in COVID-19 patients. The median time to seroconversion ranged from 10.3-11.0 days for these 3 assays. Neutralizing antibodies rose in tandem with immunoglobulin titers following symptom onset, and positive percent agreement between detection of IgG and neutralizing titers was >93%. These findings emphasize the importance of using highly accurate tests for surveillance studies in low-prevalence populations, and provide evidence that seroreactivity using SARS-CoV-2 anti-nucleocapsid protein IgG and anti-spike IgM assays are generally predictive of in vitro neutralizing capacity.

Evaluation of SARS-CoV-2 serology assays reveals a range of test performance.

Appropriate use and interpretation of serological tests for assessments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity require accurate data on assay performance. We conducted a head-to-head evaluation of ten point-of-care-style lateral flow assays (LFAs) and two laboratory-based enzyme-linked immunosorbent assays to detect anti-SARS-CoV-2 IgM and IgG antibodies in 5-d time intervals from symptom onset and studied the specificity of each assay in pre-coronavirus disease 2019 specimens. The percent of seropositive individuals increased with time, peaking in the latest time interval tested (>20 d after symptom onset). Test specificity ranged from 84.3% to 100.0% and was predominantly affected by variability in IgM results. LFA specificity could be increased by considering weak bands as negative, but this decreased detection of antibodies (sensitivity) in a subset of SARS-CoV-2 real-time PCR-positive cases. Our results underline the importance of seropositivity threshold determination and reader training for reliable LFA deployment. Although there was no standout serological assay, four tests achieved more than 80% positivity at later time points tested and more than 95% specificity.